9.9.2005

Uudet artikkelit 9.9.2005 - ISI Web of Knowledge Search Alert

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Leisure time, occupational, and commuting physical activity and the risk of stroke

Hu, G; Sarti, C; Jousilahti, P; Silventoinen, K; Barengo, NC; Tuomilehto, J

STROKE, 36 (9): 1994-1999 SEP 2005
Open Access Article available 12 months after publication

Background and Purpose - The role of physical activity, especially that of occupational and commuting physical activity, in the prediction of stroke risk is not properly established. We assessed the relationship of different types of physical activity with total and type-specific stroke risk.

Methods - We prospectively followed 47721 Finnish subjects 25 to 64 years of age without a history of coronary heart disease, stroke, or cancer at baseline. Hazard ratios (HRs) for incident stroke were estimated for different levels of leisure time, occupational, and commuting physical activity.

Results - During a mean follow-up of 19.0 years, 2863 incident stroke events were ascertained. The multivariate-adjusted ( age, sex, area, study year, body mass index, systolic blood pressure, cholesterol, education, smoking, alcohol consumption, diabetes, and other 2 types of physical activity) HRs associated with low, moderate, and high leisure time physical activity were 1.00, 0.86, and 0.74 (P-trend < 0.001) for total stroke, 1.00, 0.87, and 0.46 (P-trend = 0.011) for subarachnoid hemorrhage, 1.00, 0.77, and 0.63 (P-trend = 0.024) for intracerebral hemorrhage, and 1.00, 0.87, and 0.80 (P-trend = 0.001) for ischemic stroke, respectively. The multivariate-adjusted HRs associated with none, 1 to 29, and >= 30 minutes of active commuting were 1.00, 0.92, and 0.89 (P-trend = 0.043) for total stroke, and 1.00, 0.93, and 0.86 (P-trend = 0.028) for ischemic stroke, respectively. Occupational activity had a modest association with ischemic stroke in the multivariate analysis (P-trend = 0.046).

Conclusion - A high level of leisure time physical activity reduces the risk of all subtypes of stroke. Daily active commuting also reduces the risk of ischemic stroke.

ISSN: 0039-2499

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Effect of farming environment on sensitisation to allergens continues after childhood

Koskela, HO; Happonen, KK; Remes, ST; Pekkanen, J

OCCUPATIONAL AND ENVIRONMENTAL MEDICINE, 62 (9): 607-611 SEP 2005

Aims: The farming environment in childhood has been reported to decrease the risk of sensitisation to allergens. The purpose of the present study was to explore whether later exposure to a farming environment also could affect this sensitisation.

Methods: A population based sample of 202 women who did not live on a farm and 231 who did. The subjects filled in a questionnaire and underwent skin prick tests for several common and farming related allergens.

Results: The prevalence of sensitisation to any of the allergens was similar in the two groups (37.1 v 34.6% (p = NS). However, compared with women who did not live on a farm, the women who lived on a dairy farm showed a low prevalence of sensitisation to pollens (4.4 v 17.3%, p = 0.01) and cats (3.5 v 10.4%, p = 0.047). The risk of sensitisation to pollens and pets was lowest among women with both a childhood and adulthood farming environment and was dose dependently associated with current contact with farm animals. However, this contact increased the risk of sensitisation to bovine dander.

Conclusion: The farming environment may reduce sensitisation to common allergens also after early childhood. However, it may also increase sensitisation to farm allergens.

ISSN: 1351-0711

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Homogenization of milk has no effect on milk-specific antibodies in healthy adults

Paajanen, L; Tuure, T; Vaarala, O; Korpela, R

MILCHWISSENSCHAFT-MILK SCIENCE INTERNATIONAL, 60 (3): 239-241 2005

It has been claimed that some consumers tolerate unhomogenized cow's milk better than homogenized cow's milk. The aim of this study was to compare the effect of homogenized and unhomogenized milk on the production of specific antibodies against cow's milk proteins (casein, beta-lactoglobulin) and bovine insulin. Thirty-six milk-tolerant volunteers were challenged with homogenized and unhomogenized cow's milk for 28 days in a randomized, open, cross-over study (>400 ml of milk daily). From serum samples tak en at baseline and at the end of both milk challenges IgG and IgA were measured by ELISA against casein, beta-lactoglobulin and bovine insulin, and IgE against casein. The antibody production of the subjects remained constant for over 2 months, with no differences between the challenges with homogenized and unhomogenized milk. Inter-individual variation was greater than the intra-individual variation or the variation between the milk challenges. The order of the milk challenges did not affect the results. In conclusion, there seems to be no difference in the immunological responses to homogenized and unhomogenized milk in healthy adults with a good tolerance of milk.

ISSN: 0026-3788

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Using multilocus sequence data to define the pneumococcus

Hanage, WP; Kaijalainen, T; Herva, E; Saukkoriipi, A; Syrjanen, R; Spratt, BG

JOURNAL OF BACTERIOLOGY, 187 (17): 6223-6230 SEP 2005
Open Access Article

We investigated the genetic relationships between serotypeable pneumococci and nonserotypeable presumptive pneumococci using multilocus sequence typing (MLST) and partial sequencing of the pneumolysin gene (ply). Among 121 nonserotypeable presumptive pneumococci from Finland, we identified isolates of three classes: those with sequence types (STs) identical to those of serotypeable pneumococci, suggesting authentic pneumococci in which capsular expression had been downregulated or lost; isolates that clustered among serotypeable pneumococci on a tree based on the concatenated sequences of the MLST loci but which had STs that differed from those of serotypeable pneurnococci in the MLST database; and a more diverse collection of isolates that did not cluster with serotypeable pneumococci. The latter isolates typically had sequences at all seven MLST loci that were 5 to 10% divergent from those of authentic pneumococci and also had distinct and divergent ply alleles. These isolates are proposed to be distinct from pneumococci but cannot be resolved from them by optochin susceptibility, bile solubility, or the presence of the ply gene. Complete resolution of pneumococci from the related but distinct population is problematic, as recombination between them was evident, and a few isolates of each population possessed alleles at one or occasionally more MLST loci from the other population. However, a tree based on the concatenated sequences of the MLST loci in most cases unambiguously distinguished whether a nonserotypeable isolate was or was not a pneumococcus, and the sequence of the ply gene fragment was found to be useful to resolve difficult cases.

ISSN: 0021-9193
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Polychlorinated dibenzo-p-dioxins, dibenzofurans, and biphenyls in the general population in Finland

Kiviranta, H; Tuomisto, JT; Tuomisto, J; Tukiainen, E; Vartiainen, T

CHEMOSPHERE, 60 (7): 854-869 AUG 2005

We measured adipose tissue concentrations of polychlorinated dibenzo-p-dioxins, dibenzofurans (PCDD/Fs), and polychlorinated biphenyls (PCBs) in 420 general Finns living in southern Finland. The mean (median) concentrations of WHOPCDD/F-TEQ and WHOPCB-TEQ were 29.0 (24.1) and 20.7 (16.7) pgg(-1) fat, respectively. The concentrations clearly correlated with age. Expressing the concentrations as a function of subject's ages revealed that the exposure of Finns has declined over the last 30 years. A downward gradient was found in the concentrations from the Baltic Sea coast to inland areas in Finland, and this was assessed to be due to consumption of the Baltic Sea fish, especially Baltic herring. Linear regression models for natural logarithm WHOPCDD/F-TEQ, natural logarithm WHOPCB-TEQ, and natural logarithm WHOtotal-TEQ, explained 70%, 69%, and 72% of the variability, respectively. Age, lactation, place of residence, and fish consumption frequencies were significant predictors in the models. (c) 2005 Elsevier Ltd. All rights reserved.

ISSN: 0045-6535

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Mitochondrial DNA polymerase W748S mutation: A common cause of autosomal recessive ataxia with ancient European origin

Hakonen, AH; Heiskanen, S; Juvonen, V; Lappalainen, I; Luoma, PT; Rantamaki, M; Van Goethem, G; Lofgren, A; Hackman, P; Paetau, A; Kaakkola, S; Majamaa, K; Varilo, T; Udd, B; Kaariainen, H; Bindoff, LA; Suomalainen, A

AMERICAN JOURNAL OF HUMAN GENETICS, 77 (3): 430-441 SEP 2005
Open Access Article

Mutations in the catalytic subunit of the mitochondrial DNA polymerase g ( POLG) have been found to be an important cause of neurological disease. Recently, we and collaborators reported a new neurodegenerative disorder with autosomal recessive ataxia in four patients homozygous for two amino acid changes in POLG: W748S in cis with E1143G. Here, we studied the frequency of this allele and found it to be among the most common genetic causes of inherited ataxia in Finland. We identified 27 patients with mitochondrial recessive ataxia syndrome ( MIRAS) from 15 Finnish families, with a carrier frequency in the general population of 1:125. Since the mutation pair W748S+E1143G has also been described in European patients, we examined the haplotypes of 13 non-Finnish, European patients with the W748S mutation. Haplotype analysis revealed that all the chromosomes carrying these two changes, in patients from Finland, Norway, the United Kingdom, and Belgium, originate from a common ancient founder. In Finland and Norway, long, common, northern haplotypes, outside the core haplotype, could be identified. Despite having identical homozygous mutations, the Finnish patients with this adult-or juvenile-onset disease had surprisingly heterogeneous phenotypes, albeit with a characteristic set of features, including ataxia, peripheral neuropathy, dysarthria, mild cognitive impairment, involuntary movements, psychiatric symptoms, and epileptic seizures. The high carrier frequency in Finland, the high number of patients in Norway, and the ancient European founder chromosome indicate that this newly identified ataxia should be considered in the first-line differential diagnosis of progressive ataxia syndromes.

ISSN: 0002-9297