SYTTY
MECHANISMS OF ADVERSE HEALTH EFFECTS OF MOULDY HOUSE MICROBES: IN VITRO AND IN VIVO STUDIES ON TOXIC EFFECTS AND INFLAMMATORY RESPONSES
Project leader: Maija-Riitta Hirvonen, National Public Health
Institute (KTL), Laboratory of Environmental Microbiology, P.O.Box 95,
FIN-70701 Kuopio, Finland, tel. +358-17-201303, e-mail: Maija-Riitta.Hirvonen@ktl.fi
PUBLICATIONS
(Updated May 8, 2002)
Articles in international scientific journals
Jussila J, M. Ruotsalainen, K Savolainen, A Nevalainen and M-R Hirvonen. (1999) Nitric oxide production in human alveolar type epithelial cells induced by ?-IFN, LPS or Streptomyces anulatus. Environmental Toxicol. and Pharmacol., 7:261-266.
Hirvonen M.-R., Ruotsalainen M., Roponen M., A. Hyvärinen, T. Husman, H. Kosma V.-M. Komulainen, K. Savolainen and A Nevalainen. (1999) Nitric oxide and proinflammatory cytokines in nasal lavage fluid asssociated with symptoms and exposure to moldy house microbes. Am. J. Resp. Crit Care Med, 160:1943-1946
Huttunen K., Ruotsalainen M., Iivanainen E., Torkko P, Katila M-L. and Hirvonen M-R. (2000) Inflammatory responses and cytotoxicity in RAW264.7 macrophages caused by mycobacteria isolated from indoor air of moldy houses. Environmental Toxicology and Pharmacology, 8: 237-244.
Purokivi M., Randell J., Hirvonen M.-R. and Tukiainen H. (2000) Reproducibility of exhaled no and cytokine concentrations in induced sputum. Eur.Respir. J. 16:242-246
Suutari, M., U. Lingnell, M.-R. Hirvonen and A. Nevalainen (2000). Growth pH ranges of Streptomyces spp. ASM News 66:588-599
Hirvonen M.-R., M. Suutari, M. Ruotsalainen, U. Lignell and A. Nevalainen. (2001) Steptomyces anulatus induced inflammatory responses and cytotoxicity in macrophages are regulated by growth conditions of the microbe. Inhalation Toxicology, 13:55-68
Jussila J., Komulainen H., Huttunen K., Roponen M., Hälinen A., Hyvärinen A., Kosma V.-M, Hirvonen M.-R. (2001) Inflammatory responses in mice after intratracheal instillation of spores of Streptomyces californicus from indoor air of moldy house. Toxicology and Applied Pharmacology, 171:61-69
Murtoniemi T, Nevalainen A., Suutari M., Toivola M., Komulainen H, and Hirvonen M-R. (2001) Production of inflammatory mediators in RAW264.7 macrophages by microbes grown on six different plasterboards. Inhalation Toxicology, 13(3):233-247
Roponen M., Toivola M., Meklin T, Ruotsalainen M., Komulainen H, Nevalainen A. and Hirvonen M.-R. (2001) Differences in inflammatory responses and cytotoxicity in RAW264.7 macrophages induced by Streptomyces anulatus grown on different building materials. Indoor Air, 11(3);179-184.
Roponen M., Kiviranta J., Seuri M., Tukiainen H., Myllykangas-Luosujärvi
R. and Hirvonen M.-R. (2001) Inflammatory mediators in nasal lavage fluid,
induced sputum and serum of employees with rheumatic and respiratory disorders.
Eur.Respir. J. 18;542-548.
Huttunen K, Iivanainen E, Jussila J., Katila M-L, Hirvonen M.-R. (2001)
Comparison of the inflammatory responses of human and mice cells after
exposure to environmental mycobacteria. Inhalation Toxicology, 13:11-115.
Purokivi M., Hirvonen M.-R., Randell J., Roponen M., M. Vahteristo, Tukiainen H. (2001) Predictive value of nasal lavage compared to sputum induced samples in investigating lower respiratory tract inflammation. Eur.Respir. J.;18:951-958.
Roponen M., Seuri M., Nevalainen A., Hirvonen M.-R. (2002) Fungal spores as such do not cause nasal inflammation in mould exposure. Inhalation Toxicology, in press
Purokivi M., Hirvonen M.-R., Roponen M., Randell J., Meklin T., Tukiainen H. (2002) Changes in proinflammatory cytokines in association with exposure to microbes in moisture damages buildings. Inhalation Toxicology, in press
Murtoniemi T., Hirvonen M-R, Nevalainen A., Suutari M. (2002) Growth and cytotoxicity of microbes on six different plasterboards. Indoor Air, in press
Submitted articles
Roponen M., Seuri M., Nevalainen A., Randell J., Hirvonen M.-R. (2002)
Inflammatory mediators in nasal lavage fluid of non-symptomatic volunteers:
one year follow-up study. (Environmental Health Perspectives, submitted)
Purokivi M., Hirvonen M-R., Randell J., Roponen M., Tukiainen H.
(2001) Nitric oxide alone is an insufficient biomarker of exposure to microbes
in a moisture damaged building. (Inhalation Toxicology, submitted )
Suutari, M., U. Lingnell, M. Vahteristo, M.-R. Hirvonen and A. Nevalainen (2001). Effect of pH and nutritents on growth and sporulation of ten Streptomyces spp., isolated from moldy houses. (Appl. Envir. Microbiol, submitted)
Nielsen K.F., Huttunen, K., Hyvärinen , A., Andersen, B., Jarvis, B.B. and Hirvonen M.-R. (2001). Relationship between metabolite profiles and capability to induce cytotoxicity and produce inflammatory mediators in RAW264.7 macrophages in Stachybotrys from water-damaged buildings. (Mycopathology, submitted ).
Jussila, J., Komulainen, H., Huttunen, K., Roponen, M., Iivanainen, E., Torkko, P., Kosma, V.-M., Pelkonen, J., and Hirvonen, M.-R. (2002) Mycobacterium terrae Isolated from Indoor Air of a Moisture-Damaged Building Induces Sustained Biphasic Inflammatory Response in Mouse Lungs. (Environ. Health Perspect., submitted).
Jussila, J., Komulainen, Kosma, V.-M., Nevalainen, A., Pelkonen, J., and Hirvonen, M.-R. (2002) Spores of Aspergillus versicolor Isolated from Indoor Air of a Moisture-Damaged Building Provoke Acute Inflammation in Mouse Lungs. (Toxicol. Sci., submitted).
Jussila, J., Komulainen, H., Kosma, V.-M., Pelkonen, J., and Hirvonen, M.-R. (2002) Inflammatory Potential of the Spores of Penicillium spinulosum Isolated from Indoor Air of a Moisture-Damaged Building in Mouse Lungs. (Environ. Toxicol. Pharmacol., submitted).
Huttunen K, Hyvärinen A, Nevalainen A, Komulainen H, Hirvonen M-R. (2002). More intence production of proinflammatory mediators by indoor air bacteria than fungal spores in mouse and human cell lines. (Environmental Health Perspectives, submitted)
Holopainen M, Hirvonen M-R., Komulainen H, Klockars M. (2002). Effect of particle shape on form of silicate (mica) on particle-induced TNF-a production in mouse macrophages in vitro (submitted)
Jussila, J., Pelkonen, J., Kosma, V.-M., Mäki-Paakkanen, J., Komulainen, H., and Hirvonen, M.-R. Systemic Immunoresponses in Mice after Repeated Lung Exposure to Spores of Streptomyces californicus. (Infection and Immunity, submitted ).
Roponen M, Toivola M, Alm S, Nevalainen A, Jussila J, Hirvonen M-R.(2002)
Inflammatory and cytotoxic potential of the airborne particle material
assessed by nasal lavage and cell exposure methods. (Journal of Exposure
Analysis and Environmental Epidemiology, submitted).